| Registro completo |
| Provedor de dados: |
Biocell
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| País: |
Argentina
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| Título: |
Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
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| Autores: |
Surenciski,Mauro Rodrigo
Flachsland,Eduardo Alberto
Terada,Graciela
Mroginski,Luis Amado
Rey,Hebe Yolanda
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| Data: |
2012-04-01
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| Ano: |
2012
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| Palavras-chave: |
Cryoconservation
Liquid nitrogen
Calcium alginate
Activated charcoal
Sucrose
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| Resumo: |
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)) and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.36 n.1 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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